Skip to main content

Please note that this site in no longer active. You can browse through the contents.

Induction of Androgenesis as a Consequence of Wide Crossing in Chickpea

v1i1indu.pdf345.02 KB
Mallikarjuna, N.,( Nalini Mallikarjuna ); Jadhav, D.,( Deepak Jadhav ) ; Clarke, H. ; Coyne, C. ; Muehlbauer, F.
Journal Title: 
Journal of SAT Agricultural Research
Journal Volume: 
Journal Issue: 
Journal Pagination: 
Journal year: 

Androgenesis was observed in a wide cross of Cicer arietinum x C. pinnatifidum. Hybrids between C. arietinum x C. pinnatifidum were obtained after rescuing the hybrid embryos in vitro. The hybrids were initially devoid of any chlorophyll pigment and were albinos. Hybrid shoots were grafted onto chickpea rootstocks to obtain hybrid plant. None of the hybrid plants flowered. Flower buds were observed on the hybrid plants when 1 mg zeatin/litre was added. The flower buds were fragile, albino to semi-green, but with normal morphology. The number of divisions varied from 4 to 6. The addition of 1 mg zeatin/litre did not induce divisions in the microspores. A total of 16 hybrid plants were obtained. The number of microspores per pollen grain in an anther varied from 11 to 151 compared to more than 500 pollen grains in cultivated chickpea. The percentage of androgenic pollen grains varied from 0 to 100%. This is thought to be the first report wherein multicellularmicrospores have been consistently produced as a result of wide crossing.

Your rating: None