Method of preparation of Plant extract for Biopesticide formulation
Plants derivatives are effective and alternate sources of fungitoxic chemicals showing promising results. Laboratory to Land transfer of technology for rural farmers. It has been well recognized that some plant-derived insect-control agents could be developed into products suitable for integrated pest management, because they are selective to pests, have no or little harmful action against non-target organisms and the environment, act in many ways on various types of pest complexes and may be applied to the plant in the same way as conventional insecticides. Many plant extracts and essential oils are known to possess ovicidal, repellent and insecticidal activities against various insects. The insecticidal action of extracts of some are rapid whereas insecticidal action of some extracts are slower since over 90% mortality was only obtained at 3-4 days after treatment. These plant materials confirm their usefulness as effective insect-control agents in our field study as well. Plantation of common plants- lemon grass, Calotropis, Lantana, lemon leaves, lemon grass and neem leaf would help in the preparation of Biopesticides.
Plant Extraction Method
Soxhlet Extraction method was used for six plants, i.e., Calotropis, Lemon grass, Lemon leaves, Lantana, Moringa and Neem in which methanol was used as the solvent. At least 3 cycles of every plant sp. were carried out so as to extract all the phytochemical compounds at the temperature of 750C. Plant material except root, i.e., leaves, flowers, branches and stem were weighed and packed in filter paper as a pouchand kept in the Soxhlet. After completion of Soxhlet extraction, compound was recovered by removing methanol on rotavapour bath at 75-800 C.
Preparation of Stock Solution of Plant Extacts
The extracts were filtered using Buchner funnel and Whatman No 42 filter paper, the filtrate was concentrated to dryness under reduced pressure with a vacuum rotatry evaporator at 400C and stored under refrigeration and seal at 40C until further use. Before use, each extract was re-suspended in their respective extractant to yield 10ml extract residue per 1000 ml water From this solution, was used as pray for further use.
Submitted by Padma S Vankar on Fri, 01/01/2010 - 15:36